mouse ct26 colon carcinoma cell line  (ATCC)

Journal: Scientific Reports

Article Title: Integrated safety and microbiota profiling of fulvic acid formulations across in vitro and in vivo models

doi: 10.1038/s41598-026-37331-2

Figure Lengend Snippet: Effect of fulvic acid-containing preparations on L929 (left) and LoVo (right) cell metabolic activity after 24 h exposure. Cells were treated with MLG-50 ( A ), MLG-A50 ( B ), 3S103F Pahokee Peat Fulvic Acid Standard III ( C ), or fulvic acid ( D ) at dilutions ranging from 1:16 to 1:32768. Untreated cells serve as control (NTC). Data represent mean ± SEM from three independent experiments. Dashed lines mark the cytotoxicity threshold (CRL = 70%).

Article Snippet: The L929 mouse skin fibroblasts (CCL-1, ATCC, USA) and THP1-BlueTM NF-κB reporter cells (InvivoGen, USA) were cultured in RPMI-1640 medium (Gibco, USA), human colorectal adenocarcinoma LoVo cells (CCL-229, ATCC, USA) were cultured in DMEM/F12 medium (Gibco, USA), and human liver carcinoma HepG2 cells (HB-8065, ATCC, USA) were maintained in DMEM (Gibco, USA).

Techniques: Activity Assay, Control